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phosphorylated mlc (pmlc) ser19 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated mlc (pmlc) ser19 antibody
    Phosphorylated Mlc (Pmlc) Ser19 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+mlc+(pmlc)+ser19+antibody/anti+mlc2/pm35983865-141-2-32
    Average 90 stars, based on 1 article reviews
    phosphorylated mlc (pmlc) ser19 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Loss of Guanylyl Cyclase C (GCC) Signaling Leads to Dysfunctional Intestinal Barrier
    Article Snippet: Antibodies specific for MLC and phosphorylated MLC (serine 19) (pMLC) and STAT1 (pSTAT1) were from Cell Signaling Technology (Danvers, MA).

    Article Title: Enterocyte STAT5 promotes mucosal wound healing via suppression of myosin light chain kinase-mediated loss of barrier function and inflammation
    Article Snippet: Antibodies specific for MLC and phosphorylated MLC (serine 19) (pMLC), phospho-tyrosin694 specific STAT5 (pSTAT5), phospho-serine276 p65 (P-p65) antibodies were from Cell Signaling Technology (Danvers, MA).

    Article Title: Vincristine enhances amoeboid-like motility via GEF-H1/RhoA/ROCK/Myosin light chain signaling in MKN45 cells
    Article Snippet: The following antibodies were used: cleaved caspase-3 (#9661; Cell Signaling Technology, Beverly, MA), RhoA (ARH03; Cytoskeleton, Denver, CO), MLC (#3672; Cell Signaling Technology), phosphorylated MLC (pMLC) (#3671; Cell Signaling Technology), GEF-H1 (#4076; Cell Signaling Technology) and actin (sc-1615; Santa Cruz Biotechnology, Santa Cruz, CA).

    Blocking Assay:

    Article Title: Myosin Light Chain Kinase Mediates Intestinal Barrier Disruption following Burn Injury
    Article Snippet: .. After blocking, membranes were incubated with antibodies specific for MLCK (1∶1000, Sigma), MLC (1∶2000, Sigma), phosphorylated MLC (pMLC, 1∶1000, Cell Signaling, Beverly, MA), and β-actin (1∶5000, Sigma) overnight at 4°C. .. After wash with TBST, membranes were incubated with appropriate peroxidase-conjugated secondary antibodies (Southern Biotech, Birmingham, AL) at room temperature for 60 min.

    Article Title: Myosin light chain kinase mediates intestinal barrier disruption following burn injury.
    Article Snippet: .. After blocking, membranes were incubated with antibodies specific for MLCK (1:1000, Sigma), MLC (1:2000, Sigma), phosphorylated MLC (pMLC, 1:1000, Cell Signaling, Beverly, MA), and b-actin (1:5000, Sigma) overnight at 4uC. .. After wash with TBST, membranes were incubated with appropriate peroxidase-conjugated secondary antibodies (Southern Biotech, Birmingham, AL) at room temperature for 60 min.

    Incubation:

    Article Title: Myosin Light Chain Kinase Mediates Intestinal Barrier Disruption following Burn Injury
    Article Snippet: .. After blocking, membranes were incubated with antibodies specific for MLCK (1∶1000, Sigma), MLC (1∶2000, Sigma), phosphorylated MLC (pMLC, 1∶1000, Cell Signaling, Beverly, MA), and β-actin (1∶5000, Sigma) overnight at 4°C. .. After wash with TBST, membranes were incubated with appropriate peroxidase-conjugated secondary antibodies (Southern Biotech, Birmingham, AL) at room temperature for 60 min.

    Article Title: Myosin light chain kinase mediates intestinal barrier disruption following burn injury.
    Article Snippet: .. After blocking, membranes were incubated with antibodies specific for MLCK (1:1000, Sigma), MLC (1:2000, Sigma), phosphorylated MLC (pMLC, 1:1000, Cell Signaling, Beverly, MA), and b-actin (1:5000, Sigma) overnight at 4uC. .. After wash with TBST, membranes were incubated with appropriate peroxidase-conjugated secondary antibodies (Southern Biotech, Birmingham, AL) at room temperature for 60 min.



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    phosphorylated mlc (pmlc) ser19 antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc phosphorylated mlc (pmlc) ser19 antibody
    Phosphorylated Mlc (Pmlc) Ser19 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+mlc+(pmlc)+ser19+antibody/anti+mlc2/pm35983865-141-2-32
    Average 90 stars, based on 1 article reviews
    phosphorylated mlc (pmlc) ser19 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    phosphorylated mlc pmlc  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc phosphorylated mlc pmlc
    Involvement of ROCK in vincristine-enhanced invasive ability. A . Cells were starved in serum-free RPMI1640 for 24 h, and then treated with or without 15 μM vincristine and/or 50 μM Y27632 for 15 min. The cells were then harvested to evaluate <t>MLC</t> phosphorylation by Western blotting using anti-MLC and <t>anti-pMLC</t> antibodies. The blots in ( A ) are representative of four independent experiments. The blots were quantified by densitometry, and the results were expressed as ratio relative to the values obtained in non-treated control cells. The graph in ( A ) shows mean ± S.E. of four independent experiments. *, P < 0.05; **, P < 0.01. B . Cells on gelatin-coated coverslips were treated with drugs, fixed and stained with Alexa Fluor 488 phalloidin and DAPI. F-actin (green) and nuclei (blue) were analyzed using confocal microscopy. Arrows indicate the cells with membrane blebs. White bars, 10 μm. The graph shows means ± S.E. of three independent experiments. *, P < 0.05. C . Cells (1 × 10 6 ) were seeded into the upper chamber with or without 15 μM vincristine and/or 50 μM Y27632. After 24 h incubation, the invading cells were fixed and stained with toluidine blue. Total numbers of the stained cells were counted using a microscope. The graphs show means ± S.E. of three independent experiments. *, P < 0.05.
    Phosphorylated Mlc Pmlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+mlc+(pmlc)+ser19+antibody/Phospho-Myosin+Light+Chain+2+(Ser19)+Antibody/pmc03522013-40-23-27
    Average 96 stars, based on 1 article reviews
    phosphorylated mlc pmlc - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Involvement of ROCK in vincristine-enhanced invasive ability. A . Cells were starved in serum-free RPMI1640 for 24 h, and then treated with or without 15 μM vincristine and/or 50 μM Y27632 for 15 min. The cells were then harvested to evaluate MLC phosphorylation by Western blotting using anti-MLC and anti-pMLC antibodies. The blots in ( A ) are representative of four independent experiments. The blots were quantified by densitometry, and the results were expressed as ratio relative to the values obtained in non-treated control cells. The graph in ( A ) shows mean ± S.E. of four independent experiments. *, P < 0.05; **, P < 0.01. B . Cells on gelatin-coated coverslips were treated with drugs, fixed and stained with Alexa Fluor 488 phalloidin and DAPI. F-actin (green) and nuclei (blue) were analyzed using confocal microscopy. Arrows indicate the cells with membrane blebs. White bars, 10 μm. The graph shows means ± S.E. of three independent experiments. *, P < 0.05. C . Cells (1 × 10 6 ) were seeded into the upper chamber with or without 15 μM vincristine and/or 50 μM Y27632. After 24 h incubation, the invading cells were fixed and stained with toluidine blue. Total numbers of the stained cells were counted using a microscope. The graphs show means ± S.E. of three independent experiments. *, P < 0.05.

    Journal: BMC Cancer

    Article Title: Vincristine enhances amoeboid-like motility via GEF-H1/RhoA/ROCK/Myosin light chain signaling in MKN45 cells

    doi: 10.1186/1471-2407-12-469

    Figure Lengend Snippet: Involvement of ROCK in vincristine-enhanced invasive ability. A . Cells were starved in serum-free RPMI1640 for 24 h, and then treated with or without 15 μM vincristine and/or 50 μM Y27632 for 15 min. The cells were then harvested to evaluate MLC phosphorylation by Western blotting using anti-MLC and anti-pMLC antibodies. The blots in ( A ) are representative of four independent experiments. The blots were quantified by densitometry, and the results were expressed as ratio relative to the values obtained in non-treated control cells. The graph in ( A ) shows mean ± S.E. of four independent experiments. *, P < 0.05; **, P < 0.01. B . Cells on gelatin-coated coverslips were treated with drugs, fixed and stained with Alexa Fluor 488 phalloidin and DAPI. F-actin (green) and nuclei (blue) were analyzed using confocal microscopy. Arrows indicate the cells with membrane blebs. White bars, 10 μm. The graph shows means ± S.E. of three independent experiments. *, P < 0.05. C . Cells (1 × 10 6 ) were seeded into the upper chamber with or without 15 μM vincristine and/or 50 μM Y27632. After 24 h incubation, the invading cells were fixed and stained with toluidine blue. Total numbers of the stained cells were counted using a microscope. The graphs show means ± S.E. of three independent experiments. *, P < 0.05.

    Article Snippet: The following antibodies were used: cleaved caspase-3 (#9661; Cell Signaling Technology, Beverly, MA), RhoA (ARH03; Cytoskeleton, Denver, CO), MLC (#3672; Cell Signaling Technology), phosphorylated MLC (pMLC) (#3671; Cell Signaling Technology), GEF-H1 (#4076; Cell Signaling Technology) and actin (sc-1615; Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Phospho-proteomics, Western Blot, Control, Staining, Confocal Microscopy, Membrane, Incubation, Microscopy

    Involvement of GEF-H1 in vincristine-enhanced invasive ability. A . At 72 or 92 h after transfection of GEF-H1-specific siRNA or negative control siRNA, cells were harvested to analyze GEF-H1 expression by Western blotting using anti-GEF-H1 and anti-actin antibodies. Actin was used as a loading control. B . At 72 h after transfection, starved cells (24 h) were treated with or without 15 μM vincristine for 15 min. The cells were then lysed and analyzed by Western blotting. The blots were quantified by densitometry, and the results were expressed as a ratio relative to the values of pMLC/MLC obtained in non-treated cells. The graph shows mean ± S.E. of three independent experiments. **, P < 0.01. C . Control siRNA- or GEF-H1 siRNA-transfected cells on gelatin-coated coverslips were treated with vehicle or 15 μM vincristine. The cells were fixed and stained with Alexa Fluor 488 phalloidin and DAPI. F-actin (green) and nuclei (blue) were analyzed using confocal microscopy. Arrows indicate the cells with membrane blebs. White bars, 10 μm. The graph shows means ± S.E. of three independent experiments. **, P < 0.01. D . The number of invading cells measured by invasion assay. At 72 h after transfection, cells (1 × 10 6 ) were seeded into the upper chamber with or without 15 μM vincristine. After 24 h incubation, the invading cells were fixed, and stained with toluidine blue. Total numbers of the stained cells were counted using a microscope. The graph shows means ± S.E. of three independent experiments. *, P < 0.05.

    Journal: BMC Cancer

    Article Title: Vincristine enhances amoeboid-like motility via GEF-H1/RhoA/ROCK/Myosin light chain signaling in MKN45 cells

    doi: 10.1186/1471-2407-12-469

    Figure Lengend Snippet: Involvement of GEF-H1 in vincristine-enhanced invasive ability. A . At 72 or 92 h after transfection of GEF-H1-specific siRNA or negative control siRNA, cells were harvested to analyze GEF-H1 expression by Western blotting using anti-GEF-H1 and anti-actin antibodies. Actin was used as a loading control. B . At 72 h after transfection, starved cells (24 h) were treated with or without 15 μM vincristine for 15 min. The cells were then lysed and analyzed by Western blotting. The blots were quantified by densitometry, and the results were expressed as a ratio relative to the values of pMLC/MLC obtained in non-treated cells. The graph shows mean ± S.E. of three independent experiments. **, P < 0.01. C . Control siRNA- or GEF-H1 siRNA-transfected cells on gelatin-coated coverslips were treated with vehicle or 15 μM vincristine. The cells were fixed and stained with Alexa Fluor 488 phalloidin and DAPI. F-actin (green) and nuclei (blue) were analyzed using confocal microscopy. Arrows indicate the cells with membrane blebs. White bars, 10 μm. The graph shows means ± S.E. of three independent experiments. **, P < 0.01. D . The number of invading cells measured by invasion assay. At 72 h after transfection, cells (1 × 10 6 ) were seeded into the upper chamber with or without 15 μM vincristine. After 24 h incubation, the invading cells were fixed, and stained with toluidine blue. Total numbers of the stained cells were counted using a microscope. The graph shows means ± S.E. of three independent experiments. *, P < 0.05.

    Article Snippet: The following antibodies were used: cleaved caspase-3 (#9661; Cell Signaling Technology, Beverly, MA), RhoA (ARH03; Cytoskeleton, Denver, CO), MLC (#3672; Cell Signaling Technology), phosphorylated MLC (pMLC) (#3671; Cell Signaling Technology), GEF-H1 (#4076; Cell Signaling Technology) and actin (sc-1615; Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Transfection, Negative Control, Expressing, Western Blot, Control, Staining, Confocal Microscopy, Membrane, Invasion Assay, Incubation, Microscopy